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俄罗斯试管婴儿胚胎选取的过程

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俄罗斯试管婴儿胚胎选取的过程

时间:2019-09-06 文章来源:www.sdxyer.com作者:俄罗斯试管婴儿机构 浏览次数:

卵子取出后,与精子在试管内共同孵育,每个卵子约需10万条精子.受精后,受精卵分裂形成早期胚胎,即2~8个分裂球时即可进行胚胎移植(ET),此时约在采卵后48小时,此时间也可根据具体情况稍加以变动,如推后一天,这时也可能更利于优选胚胎.如果过早,宫腔内环境反而可能不利于接受胚胎.一般在刺激周期的前一周期,在门诊进行试验移植,以了解子宫的方位,子宫颈和子宫体间的角度及子官腔长度,并对子宫颈稍加扩张.移植时消毒外阴后,窥阴器暴露宫颈,擦净,再次用培养液擦子宫颈和穹窿及子宫颈管,将子宫颈管内粘液尽量去净.动作应尽量轻柔以减少对子宫肌肉的刺激.用特殊的移植管注入胚胎.进入宫腔,于距宫底0.5cm处注入胚胎,等待1分钟后,将头转动90°以甩掉未能滴下的一滴液体,再将导管缓慢撤出.导管取出后还要在显微镜下检查胚胎有未带出.移植后患者可以仰卧,臀部抬高,子宫很前屈者也可采取俯卧位,目的是使注入的胚胎停留在子宫腔的上方.静卧约3~6小时,可以排尿,避免尿液滞留.移植当日注射HCG5000国际单位及黄体酮30mg,以后常规每日注射黄体酮,如14天后尿HCG阴性即停止注射,妊娠者继续直到B超可见胎心后再逐步减量.对卵泡过多,可能致成卵巢过度刺激综合征者,不宜用HCG.

After the eggs are taken out, they are incubated with sperm in vitro. Each egg needs about 100,000 sperm. After fertilization, the fertilized eggs divide into early embryos, i.e. embryo transfer (ET) can be carried out when 2-8 mitotic balls are formed. At this time, about 48 hours after the eggs are picked up, the time can also be changed slightly according to specific conditions. If one day is postponed, it may also be better. Embryo selection. If it is too early, the intrauterine environment may not be conducive to embryo acceptance. Generally, in the first cycle of stimulation cycle, experimental transplantation is carried out in outpatient clinic to understand the location of the uterus, the angle between the cervix and the uterine body and the length of the sub-organ cavity, and slightly dilate the cervix. After sterilizing the vulva during transplantation, the speculum exposes the cervix, wipes it clean, and then re-expand Secondly, wipe the cervix and fornix and cervical canal with culture liquid, and remove the mucus as much as possible. The movement should be as gentle as possible to reduce the stimulation of uterine muscles. Introduce embryos into the uterine cavity with a special transfer tube. Introduce embryos 0.5 cm away from the bottom of the uterus. After a minute, turn your head 90 degrees to shake off a drop of liquid that can not be dripped, and then repeat. After the catheter is removed, the embryo is examined under a microscope. After transplantation, patients can lie on their backs, hips are raised and the uterus is prone. The purpose is to keep the implanted embryo above the uterine cavity. It can urinate for about 3-6 hours and avoid urine retention. HCG 5000 countries were injected on the day of transplantation. Progesterone and progesterone 30 mg were injected daily. If HCG was negative in urine 14 days later, progesterone would stop injecting. Pregnant women would continue to decrease gradually until fetal heart rate was visible by B-mode ultrasound. For those with excessive follicles, which may lead to ovarian hyperstimulation syndrome, HCG should not be used.


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